Blot-to-blot consistency.

Time after time.

Better blots, automatically. . .

1
  • Eliminate variations in fluid delivery and timing
  • Get reproducible results, repeatedly
  • Perform membrane processing precisely, every time
  • Program your protocol, and walk away
  • Process up to 24 membranes simultaneously
  • Get precise publication-quality results time after time.
Introducing sensible western
blot automation

Ever wish you could perform the blocking and incubation protocols precisely every time? With BlotCycler™ you can! BlotCycler™ automates solution changes, blocking, incubation of primary and secondary antibodies, and washing steps. You know – it’s the part that has to be done precisely every time, or the quality of your result suffers.

Relax, you don’t have to
change your process

With BlotCycler™, you don’t have to change your reagents or your protocols. There are no special cartridges or consumables needed. Keep using the same buffers, blocking agents and other reagents. Set up BlotCycler™  immediately, and start saving time producing better  westerns

Finally, a better way to
validate your antibodies

Up your game! BlotCycler™ is the best way to standardize your antibodies. Run up to 24 blots simultaneously using the exact same timing and fluid changes. BlotCycler™ allows you to increase productivity and get to publication-quality results faster.

Here’s proof: Better blots
when the entire assay is run at 4˚C

The following article describes how Laura Marlow at the Mayo Clinic improved specificity by processing her western blot entirely in 4˚C using BlotCycler™. With automated operation, you can do that kind of thing completely unattended.

THE PROOF

Improved Specificity of Western Blotting Results After

Processing at 4˚C Using BlotCycler™

by Laura Marlow, Mayo Clinic

Western blots with cell extract from the clear cell renal carcinoma (ccRCC) cell line were incubated with the rabbit polyclonal anti-PARP antibody (Cell Signaling Technology, Cat # 9542) using the traditional manual procedure (A) or BlotCycler™ (B).

The membranes were processed as follows: blocked with TBST-5% dry milk for 1 hour, incubated with primary antibody overnight, washed 3 x 5 minutes with TBST, incubated with the anti-rabbit secondary antibody (Jackson Immunochemicals, 1:4000 dilution) for 1 hour, and washed 3 x 15 minutes with TBST. The bands were visualized using a chemiluminescence substrate.

For the manual procedure, incubation with the primary antibody was carried out at 4°C while the rest of the procedure was performed at room temperature. For the automated procedure, incubation and all processing steps using  BlotCycler™ were done entirely at 4°C.

When the blot was processed using the manual method (A) at room temperature, multiple non-specific bands were observed. In contrast, the blot processed with BlotCycler™ (B) detected only the bands representing the uncleaved PARP protein (113 kD) as well as the C-terminal and N-terminal cleaved PARP fragments (89 kD and 24 kD respectively).

These data suggest the importance of how blocking and washing steps are performed, as well as the temperature maintained during blot washing and antibody incubation. Hands free western blot processing using BlotCycler™ enables researchers to optimize shaking, washing, and antibody incubation during western blot processing to eliminate nonspecific bands and generate high quality results.

[Fig .A] Western blots completed by traditional manual procedure - please note the nonspecific bands seen on the blot.

[Fig .A] Western blots completed by traditional manual procedure - please note the nonspecific  bands seen on the blot.

fig2

[Fig .B] Western blots processed  with BlotCycler™ show no nonspecific bands for a clearer result.

Blot-to-blot consistency

[Fig .C] (left) The first line in each blot contains a HeLa whole cell lysate, the second lane contains a recombinant human IL-2, and the third lane a mixture of HeLa whole cell lysate and hIL2

[Fig .A] Western blots completed by traditional manual procedure - please note the nonspecific bands seen on the blot.

[Fig .A] Western blots completed by traditional manual procedure - please note the nonspecific  bands seen on the blot.

fig2

[Fig .B] Western blots processed  with BlotCycler™ show no nonspecific bands for a clearer result.

Blot-to-blot consistency

[Fig .C] (left) The first line in each blot contains a HeLa whole cell lysate, the second lane contains a recombinant human IL-2, and the third lane a mixture of HeLa whole cell lysate and hIL2

Stop validating antibodies…

manually. Use BlotCycler

See it in action

This video demonstrates how BlotCycler™ processes western blots. Based on patented fluid distribution technology, the BlotCycler™ automates all steps after protein is transferred to a membrane. Simply choose the blocking time, primary antibody incubation time, secondary incubation time, and the number of washing cycles.

FAQ (frequently asked questions)

Have there been any studies comparing BlotCycler™ with manual methods?

Yes! Click on this link for a recent article:
https://precisionbiosystems.com/western-blot-automation/

Can I perform western blots overnight unattended?

Yes, simply program BlotCycler™ using one of several included programs

How many blots can BlotCycler™ do simultaneously?

Run up to 24 blots at once

Do I need to change reagents and protocols I’m currently using?

No, BlotCycler™ uses the same reagents and protocols you’re using now

What if BlotCycler™ is not for me?

We offer your money back if you’re not happy

What the Experts say about BlotCycler™

…we get a good signal at a 1:10k dilution for an antibody that gave a weak signal and lots of noise when done manually with a 1:1k dilution, and no signal at all with a 1:1k dilution using iBind”

—JM, Dartmouth College

I have been using BlotCycler™ and have been very happy with it. The machine has come in very handy as I have been able to utilize my time for other projects when I would have been washing blots or adding antibodies. I’ve set up several protocols and taught colleagues from around the department how to use BlotCycler™ and they have all been happy with it as well.

—Bryan T., Roswell Park Cancer Institute

Want better Westerns?

Walk away.

UP YOUR GAME

Start Improving your Western blot assays today!

Ask us about our 1-month FREE trial offer!

Use BlotCycler™ for free for one month.
If you are not totally impressed, we’ll take it
back for no charge! Satisfaction is 100%
guaranteed..

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About Precision Biosystems. Precision Biosystems is a Massachusetts-based company that provides solutions to help researchers automate difficult, time-consuming tasks in their laboratories. The company developed and manufactures BlotCycler™, an automated western blot processor based on patented fluid distribution technology.

Precision Biosystems
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Mansfield, MA 02048

Call: 1-888-490-4443 x 1
Fax: 1-617-812-2672
Email: hello@blotcycler.com

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